Using Aphids to Measure Turgor Pressure Inside Sieve Elements

Nick Gould*, Peter E.H. Minchin, Michael R. Thorpe

*Corresponding author for this work

    Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

    Abstract

    The phloem is the long-distance transport system for photoassimilates within the plant. The vulnerability of the phloem tissue to blockage and damage makes it technically difficult to study, which has made it one of the least understood tissues. Transport of solution through the phloem appears to be by osmotically driven bulk flow, making an understanding of phloem hydrostatic pressure important in our comprehension of phloem flow mechanics. Here we describe a method of making in vivo direct transient measurements of phloem hydrostatic pressure using excised aphid stylets to directly access the phloem.

    Original languageEnglish
    Title of host publicationMethods in Molecular Biology
    PublisherHumana Press Inc.
    Pages291-299
    Number of pages9
    DOIs
    Publication statusPublished - 2019

    Publication series

    NameMethods in Molecular Biology
    Volume2014
    ISSN (Print)1064-3745
    ISSN (Electronic)1940-6029

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