Using yeast hybrid system to identify proteins binding to small molecules

You Wang; David S. Letham; Peter C.L. John; Ren Zhang

doi:10.1007/978-1-4939-7871-7_14

Using yeast hybrid system to identify proteins binding to small molecules

You Wang, David S. Letham, Peter C.L. John, Ren Zhang^*

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

Abstract

Protein–small molecule interaction studies provide useful insights into biological processes taking place within the living cell. A special yeast hybrid system, the yeast three-hybrid method, has been developed and used to explore proteins that bind to small molecules, by which means it may be possible to unravel biological processes and dissect function of biological systems. Here we present a protocol employing this method for identifying such binding proteins.

Original language	English
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	225-234
Number of pages	10
DOIs	https://doi.org/10.1007/978-1-4939-7871-7_14
Publication status	Published - 2018

Publication series

Name	Methods in Molecular Biology
Volume	1794
ISSN (Print)	1064-3745

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10.1007/978-1-4939-7871-7_14

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Using yeast hybrid system to identify proteins binding to small molecules

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