Visual, sensitive and rapid event-specific detection of genetically modified potato EH92-527-1 by loop-mediated isothermal amplification method

Yuan Kai Tu; Yen Chun Lin; Yu Wei Feng; Yeu Yang Tseng; Han Wei Chen

doi:10.1080/09168451.2019.1661766

Visual, sensitive and rapid event-specific detection of genetically modified potato EH92-527-1 by loop-mediated isothermal amplification method

Yuan Kai Tu, Yen Chun Lin, Yu Wei Feng, Yeu Yang Tseng, Han Wei Chen^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

To date, studies on the application of loop-mediated isothermal amplification (LAMP) in the detection of genetically modified organisms (GMOs) are stably increasing and demonstrates LAMP is a potential and promising method for on spot identification of GMOs. However, little information is known for detection of GM potato events by LAMP. In this report, we developed an optimized and visual LAMP assay with high specificity and sensitivity to rapidly amplify genomic DNA of potato EH92-527-1 within 45 min. The limit of detection of LAMP in our study is 10-fold higher than the conventional PCR. Furthermore, LAMP products can be directly observed via naked eyes by addition of SYBR Green I without gel electrophoresis analysis and PCR-based equipment. Therefore, the LAMP assay developed in this paper provides an efficient, convenient and cost-effective tool for the detection of GM potato EH92-527-1.

Original language	English
Pages (from-to)	43-52
Number of pages	10
Journal	Bioscience, Biotechnology and Biochemistry
Volume	84
Issue number	1
DOIs	https://doi.org/10.1080/09168451.2019.1661766
Publication status	Published - 2020

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10.1080/09168451.2019.1661766

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title = "Visual, sensitive and rapid event-specific detection of genetically modified potato EH92-527-1 by loop-mediated isothermal amplification method",

abstract = "To date, studies on the application of loop-mediated isothermal amplification (LAMP) in the detection of genetically modified organisms (GMOs) are stably increasing and demonstrates LAMP is a potential and promising method for on spot identification of GMOs. However, little information is known for detection of GM potato events by LAMP. In this report, we developed an optimized and visual LAMP assay with high specificity and sensitivity to rapidly amplify genomic DNA of potato EH92-527-1 within 45 min. The limit of detection of LAMP in our study is 10-fold higher than the conventional PCR. Furthermore, LAMP products can be directly observed via naked eyes by addition of SYBR Green I without gel electrophoresis analysis and PCR-based equipment. Therefore, the LAMP assay developed in this paper provides an efficient, convenient and cost-effective tool for the detection of GM potato EH92-527-1.",

keywords = "EH92-527-1 potato, Event-specific detection, Genetically modified, Loop-mediated isothermal amplification",

author = "Tu, {Yuan Kai} and Lin, {Yen Chun} and Feng, {Yu Wei} and Tseng, {Yeu Yang} and Chen, {Han Wei}",

note = "Publisher Copyright: {\textcopyright} 2019 Japan Society for Bioscience, Biotechnology, and Agrochemistry",

year = "2020",

doi = "10.1080/09168451.2019.1661766",

language = "English",

volume = "84",

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issn = "0916-8451",

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T1 - Visual, sensitive and rapid event-specific detection of genetically modified potato EH92-527-1 by loop-mediated isothermal amplification method

AU - Tu, Yuan Kai

AU - Lin, Yen Chun

AU - Feng, Yu Wei

AU - Tseng, Yeu Yang

AU - Chen, Han Wei

PY - 2020

Y1 - 2020

N2 - To date, studies on the application of loop-mediated isothermal amplification (LAMP) in the detection of genetically modified organisms (GMOs) are stably increasing and demonstrates LAMP is a potential and promising method for on spot identification of GMOs. However, little information is known for detection of GM potato events by LAMP. In this report, we developed an optimized and visual LAMP assay with high specificity and sensitivity to rapidly amplify genomic DNA of potato EH92-527-1 within 45 min. The limit of detection of LAMP in our study is 10-fold higher than the conventional PCR. Furthermore, LAMP products can be directly observed via naked eyes by addition of SYBR Green I without gel electrophoresis analysis and PCR-based equipment. Therefore, the LAMP assay developed in this paper provides an efficient, convenient and cost-effective tool for the detection of GM potato EH92-527-1.

AB - To date, studies on the application of loop-mediated isothermal amplification (LAMP) in the detection of genetically modified organisms (GMOs) are stably increasing and demonstrates LAMP is a potential and promising method for on spot identification of GMOs. However, little information is known for detection of GM potato events by LAMP. In this report, we developed an optimized and visual LAMP assay with high specificity and sensitivity to rapidly amplify genomic DNA of potato EH92-527-1 within 45 min. The limit of detection of LAMP in our study is 10-fold higher than the conventional PCR. Furthermore, LAMP products can be directly observed via naked eyes by addition of SYBR Green I without gel electrophoresis analysis and PCR-based equipment. Therefore, the LAMP assay developed in this paper provides an efficient, convenient and cost-effective tool for the detection of GM potato EH92-527-1.

KW - EH92-527-1 potato

KW - Event-specific detection

KW - Genetically modified

KW - Loop-mediated isothermal amplification

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U2 - 10.1080/09168451.2019.1661766

DO - 10.1080/09168451.2019.1661766

M3 - Article

SN - 0916-8451

VL - 84

SP - 43

EP - 52

JO - Bioscience, Biotechnology and Biochemistry

JF - Bioscience, Biotechnology and Biochemistry

IS - 1

ER -

Visual, sensitive and rapid event-specific detection of genetically modified potato EH92-527-1 by loop-mediated isothermal amplification method

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