XA21-specific induction of stress-related genes following Xanthomonas infection of detached rice leaves

Nicholas C. Thomas; Benjamin Schwessinger; Furong Liu; Huamin Chen; Tong Wei; Yen P. Nguyen; Isaac W.F. Shaker; Pamela C. Ronald

doi:10.7717/peerj.2446

XA21-specific induction of stress-related genes following Xanthomonas infection of detached rice leaves

Nicholas C. Thomas, Benjamin Schwessinger, Furong Liu, Huamin Chen, Tong Wei, Yen P. Nguyen, Isaac W.F. Shaker, Pamela C. Ronald^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

The rice XA21 receptor kinase confers robust resistance to the bacterial pathogen Xanthomonas oryzae pv. oryzae (Xoo). We developed a detached leaf infection assay to quickly and reliably measure activation of the XA21-mediated immune response using genetic markers. We used RNA sequencing of elf18 treated EFR:XA21:GFP plants to identify candidate genes that could serve as markers for XA21 activation. From this analysis, we identified eight genes that are up-regulated in both in elf18 treated EFR:XA21:GFP rice leaves and Xoo infected XA21 rice leaves. These results provide a rapid and reliable method to assess bacterial-rice interactions.

Original language	English
Article number	e2446
Journal	PeerJ
Volume	2016
Issue number	9
DOIs	https://doi.org/10.7717/peerj.2446
Publication status	Published - 2016

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title = "XA21-specific induction of stress-related genes following Xanthomonas infection of detached rice leaves",

abstract = "The rice XA21 receptor kinase confers robust resistance to the bacterial pathogen Xanthomonas oryzae pv. oryzae (Xoo). We developed a detached leaf infection assay to quickly and reliably measure activation of the XA21-mediated immune response using genetic markers. We used RNA sequencing of elf18 treated EFR:XA21:GFP plants to identify candidate genes that could serve as markers for XA21 activation. From this analysis, we identified eight genes that are up-regulated in both in elf18 treated EFR:XA21:GFP rice leaves and Xoo infected XA21 rice leaves. These results provide a rapid and reliable method to assess bacterial-rice interactions.",

keywords = "EFR, RNAseq, Rice, XA21, Xanthomonas",

author = "Thomas, {Nicholas C.} and Benjamin Schwessinger and Furong Liu and Huamin Chen and Tong Wei and Nguyen, {Yen P.} and Shaker, {Isaac W.F.} and Ronald, {Pamela C.}",

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T1 - XA21-specific induction of stress-related genes following Xanthomonas infection of detached rice leaves

AU - Thomas, Nicholas C.

AU - Schwessinger, Benjamin

AU - Liu, Furong

AU - Chen, Huamin

AU - Wei, Tong

AU - Nguyen, Yen P.

AU - Shaker, Isaac W.F.

AU - Ronald, Pamela C.

PY - 2016

Y1 - 2016

N2 - The rice XA21 receptor kinase confers robust resistance to the bacterial pathogen Xanthomonas oryzae pv. oryzae (Xoo). We developed a detached leaf infection assay to quickly and reliably measure activation of the XA21-mediated immune response using genetic markers. We used RNA sequencing of elf18 treated EFR:XA21:GFP plants to identify candidate genes that could serve as markers for XA21 activation. From this analysis, we identified eight genes that are up-regulated in both in elf18 treated EFR:XA21:GFP rice leaves and Xoo infected XA21 rice leaves. These results provide a rapid and reliable method to assess bacterial-rice interactions.

AB - The rice XA21 receptor kinase confers robust resistance to the bacterial pathogen Xanthomonas oryzae pv. oryzae (Xoo). We developed a detached leaf infection assay to quickly and reliably measure activation of the XA21-mediated immune response using genetic markers. We used RNA sequencing of elf18 treated EFR:XA21:GFP plants to identify candidate genes that could serve as markers for XA21 activation. From this analysis, we identified eight genes that are up-regulated in both in elf18 treated EFR:XA21:GFP rice leaves and Xoo infected XA21 rice leaves. These results provide a rapid and reliable method to assess bacterial-rice interactions.

KW - EFR

KW - RNAseq

KW - Rice

KW - XA21

KW - Xanthomonas

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DO - 10.7717/peerj.2446

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VL - 2016

JO - PeerJ

JF - PeerJ

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M1 - e2446

ER -

XA21-specific induction of stress-related genes following Xanthomonas infection of detached rice leaves

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