Xenopus laevis Oocytes.

Stefan Bröer

doi:10.1007/978-1-60761-700-6_16

Xenopus laevis Oocytes.

Stefan Bröer^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

33 Citations (Scopus)

Abstract

Xenopus oocytes are a versatile expression system particularly suited for membrane transporters and channels. Oocytes have little background activity and therefore offer a very high signal-to-noise ratio for transporter and channel characterization. This chapter provides an overview of the basic methods used for the analysis of membrane transporters in this system, including preparation of oocytes, assays of transport activity, protocols for immunostaining and fluorescence microscopy, and other assays to study surface expression.

Original language	English
Pages (from-to)	295-310
Number of pages	16
Journal	Methods in Molecular Biology
Volume	637
DOIs	https://doi.org/10.1007/978-1-60761-700-6_16
Publication status	Published - 2010

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10.1007/978-1-60761-700-6_16

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title = "Xenopus laevis Oocytes.",

abstract = "Xenopus oocytes are a versatile expression system particularly suited for membrane transporters and channels. Oocytes have little background activity and therefore offer a very high signal-to-noise ratio for transporter and channel characterization. This chapter provides an overview of the basic methods used for the analysis of membrane transporters in this system, including preparation of oocytes, assays of transport activity, protocols for immunostaining and fluorescence microscopy, and other assays to study surface expression.",

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Xenopus laevis Oocytes.

Abstract

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