Yeast hEST1A/B (SMG5/6)- Like proteins contribute to environment-sensing adaptive gene expression responses

Xianning Lai, Traude Beilharz, Wei Chun Au, Andrew Hammet, Thomas Preiss, Munira A. Basrai, Jörg Heierhorst*

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    4 Citations (Scopus)

    Abstract

    During its natural life cycle, budding yeast (Saccharomyces cerevisiae) has to adapt to drastically changing environments, but how environmental-sensing pathways are linked to adaptive gene expression changes remains incompletely understood. Here, we describe two closely related yeast hEST1A-B (SMG5-6)-like proteins termed Esl1 and Esl2 that contain a 14-3-3-like domain and a putative PilT Nterminus ribonuclease domain. We found that, unlike their metazoan orthologs, Esl1 and Esl2 were not involved in nonsense-mediated mRNA decay or telomere maintenance pathways. However, in genomewide expression array analyses, absence of Esl1 and Esl2 led to more than two-fold deregulation of ~50 transcripts, most of which were expressed inversely to the appropriate metabolic response to environmental nutrient supply; for instance, normally glucose-repressed genes were derepressed in esl1Δ open esl2Δ open double mutants during growth in a high-glucose environment. Likewise, in a genome-wide synthetic gene array screen, esl1Δ open esl2Δ open double mutants were synthetic sick with null mutations for Rim8 and Dfg16, which form the environmental-sensing complex of the Rim101 pH response gene expression pathway. Overall, these results suggest that Esl1 and Esl2 contribute to the regulation of adaptive gene expression responses of environmental sensing pathways.

    Original languageEnglish
    Pages (from-to)1649-1659
    Number of pages11
    JournalG3: Genes, Genomes, Genetics
    Volume3
    Issue number9
    DOIs
    Publication statusPublished - 2013

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